Cultivation, identification, and application of arbuscular mycorrhizal fungi associated with date palm plants in Drâa-Tafilalet oasis

Arbuscular mycorrhizal fungi (AMF) have been frequently reported as effective tools for alleviating environmental stresses and promoting plant growth yield. In this study, we aimed to isolate, culture, identify molecular morphological AMF species associated with date palms spontaneous plants present at eight sites in the arid agroecosystem of Drâa-Tafilalet oasis Morocco. We tested capacity colonize micropropagated palm seedlings their first acclimatization stage. Soil root samples were collected propagate indigenous strains using trap culture techniques under greenhouse conditions and, same time, colonization potential was evaluated. used freshly propagated spores establish a collection single spore-derived cultures. Morphological, microscopic, approaches adopted quantify communities roots rhizosphere recovered sites. an inoculation experiment, inoculated consortium four cultured strains, alone or combination synthetic fertilizer compost. Our results showed that after two cycles culturing, frequency intensity colonizing host significantly increased, exceeding 91% 50%, respectively. Using three favorable growing helped increase rates proliferation. propagation resulted cultivation 13 strains. Molecular analyses revealed six different within our cultures: Pervetustus simplex, Claroideoglomus etunicatum, Albahypha drummondii, Septoglomus xanthium, Funneliformis mosseae, Rhizoglomus irregulare. Results experiment higher treatments augmented fertilizers than those supplemented compost 84.4% against 46.7% 26.1% 12.3%, respectively, intensity. contrast, shoot length stem diameter fertilizers. Synthetic might partially immobilized directly application, limiting availability resulting lower performance palms. These findings indicated groves are niche efficient can enhance early stages.